FDSCI 850 Food Science Graduate Seminar Seminar Outline As you develop your presentation this semester, in addition to conveying your own personal knowledge and/or professional experience about the topic you’ve selected, you will also gather, synthesize, and incorporate information from relevant peer-reviewed scientific literature related to the topic. This assignment provides you the opportunity to describe the scope of information you plan to address in your presentation by creating an outline that conveys key points you aim to discuss and connects those points to the relevant literature you’ve gathered thus far. Assignment Instructions: Prepare and submit an outline of your seminar presentation that includes the following elements. 1. Title of Seminar. List the specific title you plan to use for your presentation; the title should be concise, but also sufficiently descriptive. 2. Statement of Purpose and Overview. In a short paragraph (about 80-100 words) briefly describe the purpose (i.e., objective/goal) of your seminar and provide a narrative overview of the scope and nature of content you plan to address (that is, briefly describe what you plan to talk about). Include an explanation of how or why this topic/information is relevant to food scientists. 3. Outline of Key Points and Data/Supporting Evidence. Prepare an outline that reflects the range of information and key points you will present, beginning with the introduction/background and ending with any concluding statements. This outline should be robust, substantial, and detailed (reflecting a comprehensive summary of material you plan to discuss). a. Include at least 4-6 key points that you will address within the “body” of the presentation (i.e., “body” = content other than intro and conclusion). Identify main points and sub-points, as necessary. b. Specify any tables, figures, or other data or research findings that you plan to include in your seminar (e.g., Table X from Nutsch et al., 2023; or Figure 3 from Smith et al., 2022; etc.). c. For each key point listed in your outline, indicate (via in-text citation in APA style) which of your reference(s) provides supporting information for that point. 4. List of References. Provide a complete list of references you plan to use, formatted as an end-reference list in APA style. Refer to the “Seminar” assignment instructions for the minimum number of references required. FDSCI 850 | Food Science Graduate Seminar Seminar Outline SDS-PAGE: PRINCIPLES, PROCESS, AND APPLICATIONS [Your Name] WHAT IS SDS-PAGE? • Definition: • Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis • Purpose: • Resolution and separation of complex protein mixtures based on their differential molecular weights Source: MBL Bio SDS-PAGE: PRINCIPLE • SDS Interaction: • SDS classified as an anionic detergent, interacts with protein structures, leading to their denaturation. • This interaction disrupts the native conformation of proteins, ensuring they unfold into linear chains. • Charge Impartation: • SDS binds uniformly along the length of these protein chains, bestowing them with a consistent negative charge. • Electrophoretic Migration: • These negatively charged proteins are compelled to move towards the cathode, or the positive electrode. Source: MBL Bio HOW TO RUN/PROCESS OF SDS -PAGE • Sample Denaturation: • Initiate by amalgamating the protein sample with SDS. Subsequently, subject this mixture to a controlled heating regimen, ensuring complete denaturation and linearization of proteins. • Sample Introduction: • With utmost care, introduce the denatured protein mixture into the predefined wells of the polyacrylamide gel. This step mandates precision to prevent cross-contamination between wells. • Electrophoretic Separation: • Activate the electrophoresis apparatus, instigating the application of a calibrated electric field across the gel. • Termination: • Monitor the migration of protein bands. Upon achieving the requisite resolution and separation, cease the electrophoretic process. PROTEIN VISUALIZATION • After electrophoresis, the gel is stained (commonly with Coomassie Blue or Silver stain). • Bands represent different proteins. Figure 1. Different Bands on the Gel Source: Jovanovic et al., 2006 APPLICATIONS OF SDS-PAGE • Protein purification/determinatioj • Molecular weight determination • Protein structure analysis Source: Comis.med.uvm.edu GM VS. NON-GM PROTEIN BANDS • Band Discrepancies: • Genetically Modified (GM) proteins often manifest distinct electrophoretic patterns compared to their non-GM counterparts. • Diagnostic Utility: • SDS-PAGE serves as a diagnostic tool, enabling the confirmation or negation of specific GM protein presence. • Example: • For instance, a GM crop engineered for pest resistance might express a novel protein absent in its non-GM variant. On an SDS-PAGE gel, this would materialize as an additional band corresponding to the molecular weight of the introduced protein. CONCLUSION • Analytical Powerhouse: • SDS-PAGE stands as an indomitable methodological force in the realm of protein characterization. • Research Implications: • Its indispensability permeates various facets of molecular biology, driving both foundational research and applied scientific endeavors. REFERENCES Jovanovic, S., Barac, M., Macej, O., Vucic, T., & Lacnjevac, C. (2007). SDS-PAGE Analysis of Soluble Proteins in Reconstituted Milk Exposed to Different Heat Treatments. Sensors, 7(3), 371–383. https://doi.org/10.3390/s7030371 The principle and method of polyacrylamide gel electrophoresis (SDS-PAGE). The principle and method of polyacrylamide gel electrophoresis (SDS-PAGE) | MBL Life Sience -ASIA-. (n.d.). https://www.mblbio.com/bio/g/support/method/sdspage.html
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