Control of Gene Expression Name: ____________________________________ Group members names: ____________________________________________________________________________ ___________________________________________________________________________ Activity: What would be the action, at the level of the operon, in the following scenarios? Please do not use books or notes. Describe what is happening at the operon and whether it is regulated negatively or positively and whether its action is induced or repressed. TRP OPERON 1. You have grown E. coli on a medium high in tryptophan. Describe what is going on at the level of the operon. 2. You have grown E. coli on a medium low in tryptophan. Describe what is going on at the level of the operon. 3. Is the regulation negative or positive? 4. Is the action of the operon repressed or induced? LAC OPERON 5. You have grown P. aeruginosa on a medium of high glucose and low lactose. Describe what is going on at the level of the operon. 6. Streptococcus pneumoniae placed on a medium of low glucose and high lactose. Describe what is going on at the level of the operon. 7. S. aureus placed on a medium of high glucose and high lactose. Describe what is going on at the level of the operon. 8. S. aureus placed on a medium with neither glucose or lactose. Describe what is going on at the level of the operon. 9. Is the lac operon regulated negatively or positively? Explain 10. Is the action of the lac operon induced or repressed? 11. What do we say about the action of the glucose operon? CRISPR Name:______________________________ Group Members Names: ____________________________________________________________________________ ____________________________________________________________________________ Take the following animation link: https://www.biointeractive.org/classroom-resources/crisprcas-9-mechanism-applications In your groups, launch the interactive animation, “How It Works”. Narrate each step of the animation as if your younger sibling in middle school could understand. Homework: Watch the video “How It’s Used” and answer these question in your own words but be as detailed as possible: 1. What is CRISPR and how does it work? It evolved in bacteria to do what? How have we adapted CRISPR to work to our advantage? 2. What are some broad impacts by CRISPR therapies? How could the therapy be used by unethical means?
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