Its Gram positive rod in clusters

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My unknown bacteria is Staphylococcus Aureus because

Its Gram positive rod in clusters

Facultative anaerobic

Catalase Positive

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Results:

1. Colony morphology on TSA plate

White, circle,lobate

2. Broth turbidity result is slightly turbid.

3. Slant growth result is Echinulate, color creamy.

4. Simple stain result is

5. Gram stain result is positive purple, cocci in clusters.

6. Endospore test result is negative.

7. Media Results

-EMB No growth

-PEA good growth

-MSA No growth color yellow

-BA slightly growth white color ( alpha hemolytic)

8. Physical requirements for growth

– BHI agar result Facultative Anaerobe

– Aerobic/ Anaerobic plate result is growth in both plates.

– Temperature TSB tube result

– 0C no growth

– 25C/ room temperature Good growth

– 37C Good growth

– 55 C slightly growth